A New Paradigm for Suspension Cultures
The innovative Vertical-Wheel® impeller is the key to providing optimal fluid mixing conditions for sensitive therapeutic cells.
Rotation of the Vertical-Wheel impeller within the distinctive U-shaped vessel fully suspends and gently agitates anchorage-dependent human cells that are sensitive to a bioreactor's hydrodynamic conditions. Critically, cell culture processes and hydrodynamic conditions optimized at small scale can be consistently replicated at progressively larger volumes.
Benefits of Vertical-Wheel Mixing for Therapeutic Cells
- Complete particle suspension possible while maintaining a narrow distribution of energy dissipation rates (EDR)
- Homogenous mixing environment promotes formation of circular aggregates of similar diameters
- Minimal power input to impeller sufficient for consistent fluid flow patterns without hot zones of high turbulence and shear stress
- Kolmogrorov eddy lengths are large enough to avoid shearing effects on surface-attached cells
- Process parameters optimized at small scale can be recreated at larger volumes with predictable results
- A scalable bioreactor platform to meet your manufacturing and regulatory requirements
Consistency of Homogeneous Conditions During Scale Up
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Computational fluid dynamics (CFD) models indicate that narrow distributions of energy dissipation rates (EDR) can be achieved at various agitation rates and volumes of Vertical-Wheel bioreactors (not to scale). The reproducibility of homogenous EDR conditions is essential for optimal and scalable production of therapeutic cells, particularly those grown as aggregates.
Effects of Vertical-Wheel Hydrodynamics on Therapeutic Cells
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