A New Paradigm for Suspension Cultures
The innovative Vertical-Wheel® impeller is the key to providing optimal fluid mixing conditions for sensitive therapeutic cells.
Rotation of the Vertical-Wheel impeller within the distinctive U-shaped vessel fully suspends and gently agitates anchorage-dependent human cells that are sensitive to a bioreactor's hydrodynamic conditions. Critically, cell culture processes and hydrodynamic conditions optimized at small scale can be consistently replicated at progressively larger volumes.
Benefits of Vertical-Wheel Mixing for Therapeutic Cells
- Complete particle suspension possible while maintaining a narrow distribution of energy dissipation rates (EDR)
- Homogenous mixing environment promotes formation of circular aggregates of similar diameters
- Minimal power input to impeller sufficient for consistent fluid flow patterns without hot zones of high turbulence and shear stress
- Kolmogrorov eddy lengths are large enough to avoid shearing effects on surface-attached cells
- Process parameters optimized at small scale can be recreated at larger volumes with predictable results
- A scalable bioreactor platform to meet your manufacturing and regulatory requirements
Consistency of Homogeneous Conditions During Scale Up
Computational fluid dynamics (CFD) models indicate that narrow distributions of energy dissipation rates (EDR) can be achieved at various agitation rates and volumes of Vertical-Wheel bioreactors (not to scale). The reproducibility of homogenous EDR conditions is essential for optimal and scalable production of therapeutic cells, particularly those grown as aggregates.
Effects of Vertical-Wheel Hydrodynamics on Therapeutic Cells
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