Product Catalogue

Product Type Product Name Product Number
Bioreactors PBS-3 Vertical-Wheel Bioreactor (120 V) IA-3-B-501
PBS-3 Vertical-Wheel Bioreactor (240 V) IA-3-B-502
PBS-3 Vertical-Wheel Bioreactor, Single-Use Sensors (120 V) IA-3-B-701
PBS-3 Vertical-Wheel Bioreactor, Single-Use Sensors (240 V) IA-3-B-702
Single-Use Vessels PBS-3 Vertical-Wheel Single-Use Vessel (Floored Wheel) FA-3-D-504
PBS-3 Vertical-Wheel Single-Use Vessel (Floorless Wheel) FA-3-D-506
PBS-3 Vertical-Wheel Single-Use Vessel, Single-Use Sensors (Floored Wheel) FA-3-D-704-L
PBS-3 Vertical-Wheel Single-Use Vessel, Single-Use Sensors (Floorless Wheel) FA-3-D-706-L
Accessories PBS-3 Reusable Thermal Well IA-3-BA-006
PBS-3 Dip Tube Assembly IA-3-BA-007
PBS Autoclavable Pouch, 4" x 15" (pack of 2) IA-UNI-DA-003
PBS Single-Syringe Sampling Line, 20mL IA-UNI-DA-006
PBS Autoclavable Pouch, 8" x 18" (pack of 2) IA-UNI-DA-007
Reusable DO Sensor, 12mm OD x 220mm L with Right-Angle IC00468
Reusable pH Sensor, 12mm OD x 225mm L IC00469
60 Micron Plastic (PE) Retention Filters (pack of 5) IC01036
Software PBS-3 Vertical-Wheel Bioreactor Software 3.0 (21 CFR Part 11 cGMP Upgrade) IA-3-SW-001
Qualification Packages PBS-3 Vertical-Wheel Bioreactor IQ/OQ Protocols IA-3-BQ-004
PBS-3 Vertical-Wheel Bioreactor IQ/OQ Configuration Sheet IA-3-BQ-005

Technical Specifications:

Case Studies

Defined Biomatrices Accelerate MSC Manufacturing Across Scales
Overcoming Bioprocess Bottlenecks in the Large-Scale Expansion of High-Quality hiPSC Aggregates in Vertical-Wheel Stirred Suspension Bioreactors
Initial Power Measurements for a Family of Novel Bioreactors

User Resources

PBS-3 Single-Use Bioreactor User Manual (Single-Use Sensors, v4 Software) [P/Ns: IA-3-B-711 & -712]
PBS-3 Single-Use Bioreactor User Manual (Reusable Sensors, v4 Software) [P/Ns: IA-3-B-511 & -512]
PBS-3 Datasheet


What is the role of the 3L Vertical-Wheel bioreactor in process scale up?
faq icon

Successful transition of a small scale cell culture process designed in PBS Minis into the 3L bioreactor represents the first step in achieving true scalability of manufacturing therapeutic cells. The 3L can meet production needs for early stage clinical studies and also serves as stepping stone for continued scale up into the 15L bioreactor.

What is the difference between floored and floorless Vertical-Wheel impellers and what kind of cell culture processes are each suited for?
faq icon

The floored version has more panels around the circumference of the Vertical-Wheel that create cup-like sections and is better suited for cells grown on microcarriers or as single cells. The floorless version is nearly identical but with less panels and allows more fluid flow towards the axle, and is better suited for cell aggregate applications. Both versions have nearly identical hydrodynamic performance.

Are single-use dissolved oxygen and pH sensors available for use with all Vertical-Wheel bioreactors?
faq icon

The 3L, 15L, and 80L bioreactors are available in one version that accommodates single-use sensors and another version that is designed for reusable sensors (no cross-compatibility). The housing for the reusable sensor version can be modified to solely accommodate single-use sensors; please contact our Applications Engineering team for the service details.

What are the recommended sampling and harvesting methods?
faq icon

For Mini vessels, the cap on top can be unscrewed for pipetting in a biosafety hood. For the 3L bioreactor, media can be withdrawn from a sample line connected to the vessel via an automated pump or manually. For the 15L and 80L bioreactors, the adjustable height harvest valve can fulfill both functions.

How can I monitor process parameters during a cell culture run?
faq icon

The 3L bioreactor’s software allows for customizable programming of process controls and real-time monitoring of parameters using various sensors. Feedback loops can be used to pause a run based on deviations from a set range.

Can I control and monitor my cell culture process remotely?
faq icon

Yes, the software on 3L and larger size bioreactors allows for remote process monitoring, adjustments, and alarm reporting over a secure connection to various devices.

How is process-related data stored and accessed?
faq icon

The software can customize how often and what kind of data will be stored on the bioreactor’s on-board hard drive. Automatic downloading of data can be directed to a local computer with direct LAN connection. All user logins, process changes, and data histories follow 21 CFR Part 11 requirements for cGMP.

Can the 3L Vertical-Wheel bioreactor fit on top of a standard size lab bench?
faq icon

Yes, the 3L bioreactor is designed to be place on a lab bench, rolling cart, or similar platform. Technical specifications such as dimensions and weight are available on the 3L product page.

How do I know the number and types of Vertical-Wheel bioreactors I will need?
faq icon

Please use the Contact form to describe your process requirements and our Applications Engineering team will be able to assist with your planning.

I have other questions regarding usage or troubleshooting of the PBS Mini.
faq icon

Please use the Products and/or Service quote form to submit any questions or inquiries related to a specific product(s).

Product and Service Request Form

Please use this form for quotes related to specific product(s) and/or service and we will respond within the next business day.

What products/services would you like to discuss? (select all that apply)
Current Process Stage
Cell type(s) of interest (select all that apply)
How did you hear about PBS Biotech?
General comments (optional)
Thank you! Your submission has been received!
Oops! Something went wrong while submitting the form.