Cell Therapy Vertical-Wheel Bioreactor Scaling Calculator

Optimize your agitation rates and narrow distributions of energy dissipation rates (EDR) across scales (from 60mL to 80L)

How it Works

The PBS Biotech team has performed computational fluid dynamics (CFD) modeling to develop a method to translate agitation speeds across the different sized vessels. The CFD modeling shows that important volume average hydrodynamic variables can be maintained throughout scale-up (Dang, Borys 2021). These equations allow operators to define a working range of hydrodynamic variables at one scale and calculate the corresponding agitation rates at other modelled scales (Dang, Borys 2021).

  • The suggested operating range of agitation rates was determined for successful culture and scale up of iPSC aggregates using the graph  shown above
  • For optimal results, stay within operational window when scaling up to large systems
  • To view scale-up results: enter your working volume, and your desired agitation rates

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