PBS-0.5 Mini Perfusion Single-Use Vessel

Recommended for working cell culture volumes ranging from 400-500mL

Efficiently Scale Up Perfusion-based PSC Cultures

The PBS 0.5L Single-Use Vessel with PSC Perfusion Filter (60 µm) empowers researchers to scale up perfusion-based PSC cultures efficiently maintaining cell quality and process control from small-scale experiments to clinical production.

Key Advantages for Your Cell Therapy

Engineered for Controlled Perfusion: Achieve uniform PSC aggregates and superior differentiation results through consistent medium exchange, supporting high yields in regenerative medicine and cell therapy workflows
Integrated Sterile System: The built-in 60 µm cell retention filter with dedicated feed and waste lines ensures contamination-free, continuous perfusion—ideal for testing multiple feeding or media optimization strategies
Flexible and Pump-Ready: Designed with both standard and Masterflex® High-Performance Precision Pump silicone tubing for direct compatibility with leading peristaltic pumps. C-Flex® tubing supports sterile welding and extended line configurations for easy system integration
Seamless Bioreactor Compatibility: Pairs perfectly with the PBS-Mini Vertical-Wheel Bioreactor Base Unit (P/N: FA-UNI-B-501) to provide a compact, scalable solution for advanced perfusion research and process development

PBS Bioreactors vs. Stirred Tank Bioreactors

Feature	Vertical-Wheel Bioreactor	Stirred Tank Bioreactor
Mixing Mechanism	Vertical impeller that generates a gentle, large-scale, upward-and-outward flow.	Horizontal impeller that creates high-shear zones and turbulent eddies.
Shear Stress	Very low. Designed to minimize hydrodynamic stress, making it ideal for shear-sensitive cells like stem cells and cell aggregates.	High, especially near the impeller blades. Can be detrimental to fragile cells, although impeller designs have been optimized to reduce this.
Scalability	Linear. The hydrodynamic conditions remain consistent as the volume increases, allowing for predictable scale-up.	Complex. Scaling up requires careful optimization to maintain consistent conditions, as fluid dynamics change with volume.
Homogeneity	Excellent. The vertical wheel creates a highly uniform environment with eliminating occurrences of high turbulence.	Good. Requires careful design to ensure uniform mixing and prevent gradients, especially in large vessels.
Applications	Primary: Shear-sensitive cell cultures (hiPSCs, MSCs, T-cells), viral vectors, and exosomes	Primary: Large-scale fermentation (bacteria, yeast), and general mammalian cell culture (e.g., CHO cells for biologics).

Product Parts and Technical Specifications

Product Type	Product	Product Number
Base Unit	PBS-Mini Vertical-Wheel Bioreactor Base Unit (120-240 V)	FA-UNI-B-501	Request a quote
Single-Use Vessels	PBS 0.5 Single-Use Vessel with 60μm PSC Perfusion Filter (pack of 1)	A-0.5-D-201	Request a quote
Accessories	PBS-Mini Extended 4-Gang DC Power Distribution Cable	IA-UNI-BA-112	Request a quote
	60 Micron Plastic (PE), Retention filter (5-pack)	IC01036	Request a quote
	PBS-3 Dip Tube Assembly (pack of 1)	IA-3-BA-007	Request a quote

Working volume range:	400-500mL (500mL recommended)
Number of ports	2
Gassing	Diffusion through 0.22 micron filter on vent cap
Liquid transfer	Port with screw-on vent cap
Product contact materials	Meet requirements for USP Class VI Testing for Plastics and/or ISO 10993

Vertical-Wheel Bioreactor
Scaling Calculator

Vertical-Wheel Bioreactor
Support Portal

Vertical-Wheel Bioreactor
Product Guides and Manuals

Single-use bioreactor company of the year 2025

The PBS Mini Bioreactor 90 Day Guarantee

If you are not satisfied with the performance of the PBS-Mini products or accessories get your money back. Must show necessary performance documentation to qualify.