Achieving Clinically Relevant Yields of High-Potency CAR-NK Cell Expansion in PBS Vertical-Wheel Bioreactors through Integrated Perfusion Via Xcell ATF from GeneFab and Repligen

Introduction

This white paper highlights a collaboration between PBS Biotech, GeneFab, and Repligen to demonstrate a closed-system process workflow for natural killer (NK) cell expansion and scale-up using PBS Vertical-Wheel Bioreactors (VWBs) at 100mL and 3L working volumes, with batch, fed-batch, and perfusion medium exchange strategies using Alternating Tangential Flow-2 (ATF2) device to achieve a clinically relevant yield of highly potent NK cells.

Key Takeaways

  • 2.5X higher NK cell potency achieved using PBS Vertical-Wheel Bioreactors (VWBs) compared to the G Rex platform
  • VWBs provide linear scalability, workflow flexibility, and bioprocess control required for rapid and reproducible scale-up for manufacturing
  • 2.6X greater cell yield during expansion with perfusion media exchange in PBS 3 VWBs vs. fed-batch methods

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