The Resource Library

This brochure introduces PBS MiniPRO as an automated, small-scale Vertical-Wheel® Bioreactor system for cell therapy process development. The platform provides dissolved oxygen and pH control, closed-system operation, perfusion-capable workflows, and parallel operation. As a scale-down model of PBS manufacturing-scale Bioreactors, MiniPRO supports predictive process development and technology transfer. The brochure highlights improved process control, reduced variability, streamlined scale-up, and support for workflows requiring consistent aggregate formation and gentle mixing conditions.

This poster reviews key process development considerations required to achieve robust, efficient, and scalable manufacturing of allogeneic cell therapies**.** The authors discuss major scale-up challenges including equipment selection, hydrodynamic control, oxygen transfer, medium exchange, and cell harvest operations. Case studies demonstrate how process optimization and Vertical-Wheel bioreactor technology can reduce seed train requirements, shorten manufacturing timelines, decrease facility footprint, and integrate closed-system operation. The work also presents methodologies for…

This poster evaluates the PBS MiniPRO as a controlled scale-down platform for human induced pluripotent stem cell process development. The system combines four 500 mL Vertical-Wheel vessels with online pH and dissolved oxygen monitoring, gas control, integrated retention filters, perfusion pumps, and closed fluid-handling connections. Experiments examined process reproducibility, direct thaw from high-density cell banks, seed train, commercial and modified media, settlement and perfusion feeding, oxygen control, aggregate morphology, and sampling methods. Controlling dissolved oxygen at 50%…

This brochure introduces the PBS-0.5 Vertical-Wheel® PSC Perfusion Vessel, an integrated perfusion solution for pluripotent stem cell aggregate cultures and other large-particle processes. Continuous medium exchange through an integrated retention filter can support aggregate homogeneity, reduce manual handling, and enable downstream differentiation workflows. Comparative data show tighter aggregate-size distributions and smaller average aggregate diameters with perfusion than settlement-based medium exchange. The brochure also covers scalability, equipment compatibility, ordering…

The PBS Closed-System Sampling Conical supports aseptic, representative sample collection from PBS Vertical-Wheel® Bioreactors while maintaining process integrity and sample quality. The single-use assembly combines a gamma-irradiated vented conical, integrated dip tube, and weldable tubing to reduce contamination risk, handling steps, and operator variability. Compatible with stem cells, immune cells, organoids, and aggregate cultures, it supports routine in-process analyses including cell counts, viability testing, metabolite monitoring, and aggregate characterization.

This poster integrates high-density working cell banks with a computer-controlled parallel Vertical-Wheel® Bioreactor system for human induced pluripotent stem cell process development. Banks generated through three-dimensional expansion were thawed directly into suspension, reducing multiple adherent seed-train stages before differentiation. Post-thaw cultures maintained reproducible growth, OCT4 expression, normal karyotype, and functional pluripotency. The scale-down system provided dissolved oxygen and carbon dioxide control, perfusion feeding, and manufacturing-relevant hydrodynamics.…

This poster presents a closed iPSC manufacturing workflow integrating carrier-free aggregate perfusion, in-vessel harvest, concentration, formulation, automated fill-finish, and cryopreservation. A 3 L Vertical-Wheel® Bioreactor was compared with a 0.1 L scale-down process. Both achieved greater than 54-fold expansion, greater than 97% viability, and aggregate diameters of approximately 530 µm. The 3 L process generated approximately 3.7 billion viable cells, with 100% recovery after harvest and 95% post-harvest viability. Post-thaw studies assessed morphology, growth, pluripotency, and…

This flyer describes the PBS Biotech CDMO Partner Program, which provides flexible access to PBS-3 and MiniPRO Vertical-Wheel® Bioreactor systems through lease-to-own, installment, and on-demand acquisition models. The program helps CDMOs, CMOs, and process-development organizations expand cell therapy capabilities while reducing upfront capital requirements. Participants receive training, onboarding, technical support, and commercial collaboration opportunities. Program structures, deployment options, partner tiers, and qualification pathways are designed to support manufacturing capacity…