Aggregate Perfusion Medium Exchange in Vertical-Wheel Bioreactors for Scalable PSC Production

Introduction
Scale-up of quality assured pluripotent stem cells (PSCs) to meet increasing demand for lifesaving therapies is heavily studied in cell therapy bioprocessing.  While emphasis has been placed on the development of large-scale bioreactor technologies, success hinges on the ability to identify key process parameters that impact the yield and quality of cells at large scale.

•  Settling method for small scale MX
• Impact of settling on aggregate culture homogeneity
• Scalable MX using perfusion
• Impact of perfusion MX on culture heterogeneity