Aggregate Perfusion Medium Exchange in Vertical-Wheel Bioreactors for Scalable PSC Production
Introduction
Scale-up of quality assured pluripotent stem cells (PSCs) to meet increasing demand for lifesaving therapies is heavily studied in cell therapy bioprocessing. While emphasis has been placed on the development of large-scale bioreactor technologies, success hinges on the ability to identify key process parameters that impact the yield and quality of cells at large scale.
Topics covered in this note
- Settling method for small scale MX
- Impact of settling on aggregate culture homogeneity
- Scalable MX using perfusion
- Impact of perfusion MX on culture heterogeneity
