PBS-0.1 Mini Vertical-Wheel Bioreactor

Recommended for working cell culture volumes
ranging from 60-100mL

The Best Starting Point for Your Cell Therapy Journey

The PBS-0.1 Mini^® single-use bioreactor is designed for early stage cell therapy development, supporting 60 to 100 mL working volumes and incorporating Vertical-Wheel^® mixing for gentle, efficient, and scalable culture conditions. This makes it an ideal platform for optimizing small-scale processes with a clear path for linear scale up to larger Vertical-Wheel bioreactors.

Flexible platform: a universal base unit supports PBS-0.1, PBS-0.5, and PBS-0.5 Mini Perfusion single-use vessels, enabling smooth process scalability.
GMP readiness: all product-contact materials comply with regulatory standards for use in first-in-human clinical trials.
Sterile handling: removable vessel caps allow straightforward aseptic liquid transfer in a biosafety cabinet.

“The scale-down PBS-0.1 Mini provided a cost-effective platform to de-risk decisions for early-stage development, enabling the identification of key process parameters that were easily transferred to the PBS-3.”
-Excerpt from GeneFab and Repligen Case Study-

PBS-0.1 Mini vs. Stirred Tank Bioreactors

Feature	Vertical-Wheel Bioreactor	Stirred Tank Bioreactor
Mixing Mechanism	Vertical-Wheel impeller that generates gentle and scalable fluid conditions.	Horizontal impeller that creates wide range of turbulent eddies and zones of highly variable flow.
Hydrodynamic Conditions	Homogeneous fluid flow throughout the vessel, meaning all cells experience similiar growth conditions.	Heterogeneous, with cells experiencing variable conditions depending on location within vessel.
Shear Stress	Consistently low throughout the environment, which is ideal for shear-sensitive cells such as those on microcarriers or grown as aggregates.	High, especially near tips of impeller blades, which can be detrimental to shear-sensitive cells.
Scalability	Linear, with reproducible hydrodynamic conditions at all volumes thus allowing for predictable scale-up.	Complex, with scale up requiring careful optimization to maintain consistent conditions.
Applications	Primarily larger particles (aggregates, microcarriers) of adherent, shear-sensitive cells (hiPSCs, MSCs, T-cells).	Primarily single cells for mammalian (CHO for biologics) or fermentation (bacterial, yeast) cultures.

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Vertical-Wheel Bioreactor
Scaling Calculator

Vertical-Wheel Bioreactor
Support Portal

Vertical-Wheel Bioreactor
Product Guides and Manuals

Product Parts and Technical Specifications

Product Type	Product	Product Number
Base Unit	PBS-Mini Vertical-Wheel Bioreactor Base Unit (120-240 V)	FA-UNI-B-501	Request a quote
Single-Use Vessels	PBS-0.1 Vertical-Wheel Single-Use Vessel with Bottom Port (pack of 1)	FA-0.1-D-101	Request a quote
Single-Use Vessels	PBS-0.1 Vertical-Wheel Single-Use Vessel (pack of 4)	FA-0.1-D-001	Request a quote
Accessories	PBS-Mini Extended 4-Gang DC Power Distribution Cable	IA-UNI-BA-112	Request a quote

Working volume range:	20 - 100 mL (For liquid mixing) 60 - 100 mL (For cell culture)
Number of ports	1
Gassing	Diffusion through 0.22 micron filter on vent cap
Liquid transfer	Port with screw-on vent cap
Product contact materials	Meet requirements for USP Class VI Testing for Plastics and/or ISO 10993

Download Full Technical Spec Sheet