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Suspension Culture on Microcarriers and as Aggregates Enables Expansion and Differentiation of Pluripotent Stem Cells (PSC)

Introduction

Human pluripotent stem cells (PSCs) hold a great promise for promoting regenerative medical therapies due to their ability to generate multiple mature cell types and for their high expansion potential. However, cell therapies require large numbers of cells to achieve desired therapeutic effects, and traditional two-dimensional static culture methods cannot meet the required production demand for cellular therapies. One solution to this problem is scaling up expansion of PSCs in bioreactors using culture strategies such as growing cells on microcarriers or as aggregates in suspension culture.

Topics covered in this note

• PSCs grown on microcarriers and as aggregates retain typical PSC cellcycle profiles and morphology
• PSCs grown on microcarriers and as aggregates show differentiation potential similar to 2D cultures
• PSCs cultured in 3D suspension can be effectively differentiated into neural progenitor cells and cardiomyocytes