Suspension culture improves iPSC expansion and pluripotency phenotype
Introduction
Induced pluripotent stem cells (iPSCs) offer potential to revolutionize regenerative medicine as a renewable source for islets, dopaminergic neurons, retinal cells, and cardiomyocytes. However, translation of these regenerative cell therapies requires cost-efficient mass manufacturing of high-quality human iPSCs.
Topics covered in this note
- Generation of iPSC lines from human peripheral blood mononuclear cells
- 3D suspension conditions induce transcriptional changes without promoting copy number variations
- iPSCs expanded using 3D suspension conditions generate more mature teratomas with fewer proliferative cells
