Expansion of PSC Aggregates in the PBS-Mini Vertical-Wheel Bioreactor

Introduction
This protocol details a scalable workflow for generating and expanding pluripotent stem cell (PSC) aggregates, beginning with 2D adherent seed cultures in T-flasks and transitioning to dynamic 3D suspension growth in PBS-Mini Vertical-Wheel^® bioreactors. Designed for reproducibility and translational relevance, the framework enables precise control over aggregate formation, viability, and proliferation—laying a robust foundation for downstream applications in regenerative and cell-based therapies.