Overcoming Scale-Up Challenges in iPSC Production
This case study evaluates an integrated workflow for expansion, dissociation, harvest, concentration, and serial passage of human induced pluripotent stem cells using Vertical-Wheel® Bioreactors and single-use tubular-bowl centrifugation. Closed processing achieved 89.9% cell recovery compared with 93.3% for open benchtop centrifugation, with similar post-processing growth. Serial expansion at 3 L consistently achieved greater than 90% recovery and greater than 95% post-harvest viability while maintaining spherical aggregates, stable expansion, and OCT4 and SOX2 expression. Process modeling from 60 mL to 80 L supports scalable, low-shear downstream processing of iPSC cultures.
This guide collects and summarizes process parameters from successful experiments performed in Vertical-Wheel® bioreactors, across a variety of cell types including pluripotent stem cells, mesenchymal stem/stromal cells, exosomes and extracellular vesicles, organoids, and immune cells. Find the publication or resource most relevant to your own experiment to help with your process development.
This protocol provides step-by-step guidance for forming and expanding pluripotent stem cell aggregates in PBS-MINI Vertical-Wheel® bioreactors. The workflow begins with thawing and adherent seed-train culture in T-flasks, followed by transfer to three-dimensional suspension culture in 0.1 L or 0.5 L vessels. Instructions cover inoculation, medium exchange, sampling, morphology assessment, metabolite and pH analysis, cell counting, viability testing, and harvest in the PBS-Mini bioreactor. The document presents recommended starting parameters for seeding density, working volume, agitation…
This publication reports a fully suspended process for expansion and six-stage differentiation of human induced pluripotent stem cells into stem cell-derived islets in 0.1 L and 0.5 L Vertical-Wheel® Bioreactors. A fivefold increase in vessel volume produced a twelvefold increase in islet-equivalent yield without compromising islet structure. The 27-day process generated endocrine populations expressing ?-, ?-, and ?-cell markers with glucose-responsive insulin secretion and transcriptomic characteristics approaching adult islets. The study also evaluated donor-derived iPSC lines, cellular…
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