Scalable CAR-NK Cell Expansion Using Vertical-Wheel Bioreactors with XCell ATF Perfusion

The combination of PBS Biotech’s Vertical-Wheel^® Bioreactor and Repligen’s XCell^® Alternating Tangential Flow filtration delivers a closed and scalable low-shear environment optimized for high-quality immune-cell production. This application note investigates nutrient-delivery and perfusion-feeding approaches for high-potency expansion of chimeric antigen receptor natural killer (CAR-NK) cells at a 3L working scale.