Therapeutic Proteins Application
Since the dawn of the biotech era in the early 1980s, therapeutic proteins have constituted a majority of biological drugs produced in the world. Some of the earlier drugs that were approved used anchorage-dependent cells, but most of the recent biological drugs has been produced in robust animal cell lines that have been adapted over time to grow in single cell suspension, such as commonly used Chinese Hamster Ovary (CHO) cells.
In this field, processes are typically optimized to culture cells to very high densities involving complex media and multiple nutrient feeds at small scale and then scaled up to larger working volumes. Oftentimes, inconsistencies in cell culture performance arise during process scale-up due to differences in the impeller and vessel geometries of the bioreactors, which can result in different shear stress and foam profiles. The Vertical-Wheel™ Bioreactor System from PBS Biotech can help alleviate this scale-up issue by offering a consistent Vertical-Wheel™ impeller and vessel shape design from 0.1 L to 500 L scales.
A fed-batch process involving a hybridoma cell line expressing a monoclonal antibody (mAb) has been successfully scaled up from 3 L to 500 L scale in the Vertical-Wheel™ Bioreactor System, where agitation rates in the larger bioreactors were set by maintaining the same impeller power input per volume (P/V) as the 3 L. Comparable viable cell densities and protein titers were achieved across all bioreactor sizes (PBS 3, PBS 15, PBS 80, and PBS 500).
A high density fed-batch process using lactate-adapted CHO DG44 cells has also successfully been cultured in 3L and 15L Vertical-Wheel™ Bioreactor Systems, showing comparable peak viable cell density of 25 million cell/mL and >90% viability was maintained through 12 days of culture.
PBS Biotech is continually working to generate cutting-edge results in the Vertical-Wheel™ Bioreactor System for Therapeutic Protein application, including more challenging processes that involve continuous medium exchange with perfusion devices to push cell densities even further.
For this particular application, PBS Biotech generally recommends the AirDrive option of the Vertical-Wheel™ Bioreactor System.
Since the dawn of the biotech era in the early 1980s, therapeutic proteins have constituted a majority of biological drugs produced in the world. Some of the earlier drugs that were approved used anchorage-dependent cells, but most of the recent biological drugs has been produced in robust animal cell lines that have been adapted over time to grow in single cell suspension, such as commonly used Chinese Hamster Ovary (CHO) cells.
In this field, processes are typically optimized to culture cells to very high densities involving complex media and multiple nutrient feeds at small scale and then scaled up to larger working volumes. Oftentimes, inconsistencies in cell culture performance arise during process scale-up due to differences in the impeller and vessel geometries of the bioreactors, which can result in different shear stress and foam profiles. The Vertical-Wheel™ Bioreactor System from PBS Biotech can help alleviate this scale-up issue by offering a consistent Vertical-Wheel™ impeller and vessel shape design from 0.1 L to 500 L scales.
A fed-batch process involving a hybridoma cell line expressing a monoclonal antibody (mAb) has been successfully scaled up from 3 L to 500 L scale in the Vertical-Wheel™ Bioreactor System, where agitation rates in the larger bioreactors were set by maintaining the same impeller power input per volume (P/V) as the 3 L. Comparable viable cell densities and protein titers were achieved across all bioreactor sizes (PBS 3, PBS 15, PBS 80, and PBS 500).
A high density fed-batch process using lactate-adapted CHO DG44 cells has also successfully been cultured in 3L and 15L Vertical-Wheel™ Bioreactor Systems, showing comparable peak viable cell density of 25 million cell/mL and >90% viability was maintained through 12 days of culture.
PBS Biotech is continually working to generate cutting-edge results in the Vertical-Wheel™ Bioreactor System for Therapeutic Protein application, including more challenging processes that involve continuous medium exchange with perfusion devices to push cell densities even further.
For this particular application, PBS Biotech generally recommends the AirDrive option of the Vertical-Wheel™ Bioreactor System.