PBS Product Family
A key advantage of PBS bioreactors is the uniform and low shear mixing performance they provide, especially at larger working volumes targeting commercial manufacturing scales.
In the PBS bioreactor system, the size of the Vertical-Wheel™impeller increases proportionally with bioreactor volume, such that the highest shear stress inside the bioreactor (typically around the impeller region) remains roughly constant as determined by computational fluid dynamics (CFD) analysis. Furthermore, the minimum Kolmogorov eddy length, a hydrodynamic parameter linked to cell damage, is calculated in PBS bioreactors to be above the threshold size that is considered to be damaging to MC cultures, at typical agitation rates required for MC suspension.
Additionally, the inner surfaces are designed to eliminate wrinkles and crevices where particles such as cell clumps and MCs may accumulate. For heat-sealed PVDF film-based bioreactor bags, the walls align precisely with the shape of the housing that holds the bags, preventing the formation of creases at bag installation. The smooth surface in a round bottom shape allows efficient particle (MCs) suspension along with the sweeping power of the tangential flow of liquid created by the vertical impeller. This unique feature is applicable from small size to the larger single-use bioreactor bag systems.
For more details on how to operate a PBS bioreactor system in a cell culture application, please refer to this instructional video authored by staff at Johns Hopkins University for the PBS 3 AIR system and available for viewing at the Journal of Visualized Experiments (JoVE) website.
In the PBS bioreactor system, the size of the Vertical-Wheel™impeller increases proportionally with bioreactor volume, such that the highest shear stress inside the bioreactor (typically around the impeller region) remains roughly constant as determined by computational fluid dynamics (CFD) analysis. Furthermore, the minimum Kolmogorov eddy length, a hydrodynamic parameter linked to cell damage, is calculated in PBS bioreactors to be above the threshold size that is considered to be damaging to MC cultures, at typical agitation rates required for MC suspension.
Additionally, the inner surfaces are designed to eliminate wrinkles and crevices where particles such as cell clumps and MCs may accumulate. For heat-sealed PVDF film-based bioreactor bags, the walls align precisely with the shape of the housing that holds the bags, preventing the formation of creases at bag installation. The smooth surface in a round bottom shape allows efficient particle (MCs) suspension along with the sweeping power of the tangential flow of liquid created by the vertical impeller. This unique feature is applicable from small size to the larger single-use bioreactor bag systems.
For more details on how to operate a PBS bioreactor system in a cell culture application, please refer to this instructional video authored by staff at Johns Hopkins University for the PBS 3 AIR system and available for viewing at the Journal of Visualized Experiments (JoVE) website.
