Cell Therapies Application
Stem cell therapy research has rapidly grown in interest in recent years, as advanced treatments based on stem cells give the potential to cure a wide variety of serious diseases and infirmities, ranging from inflammation to fixing spinal cord injuries. Numerous clinical products are currently in trial, but the clinical batches to date have mostly been cultured on 2-dimensional systems such as T-flasks, which carry significant economic and regulatory drawbacks for scale up.
One of the scalable approaches for large volume production of stem cells is to grow them on microcarriers (MCs) suspended in a bioreactor as a 3D culture to utilize its entire volume and provide more uniform culturing conditions for the cells to maintain cell homogeneity. The Vertical-Wheel™ Bioreactor System offers an advantage to other bioreactor systems in its ability to keep the MCs in suspension during culture at low agitation rate due to the vertically-oriented wheel, while imparting low shear stress to the cells with gentle mixing owing to the large surface area of the impeller that allows cells to attach quickly and evenly on the MCs.
Bone-Marrow Derived Human Mesenchymal Stem Cells (BM-hMSCs), a commonly-used stem cell type for research and clinical trials, has been successfully cultured in the PBS 3 Vertical-Wheel™ Bioreactor System to very high cell densities:
Scalability of the Vertical-Wheel™ design has also been demonstrated using hMSCs and cell culture medium from Rooster Bio, resulting in consistently high growth rate (1.7-1.8 day doubling time) in PBS 0.5 and PBS 3 systems. The PBS 0.1 and PBS 0.5 MINI Bioreactor systems are ideal for early-stage process development, as they require smaller quantities of expensive reagents to perform screening studies.
PBS Biotech is continually working to generate cutting-edge results in the Vertical-Wheel™ Bioreactor System for Cell Therapy application, including process scale-up to larger working volumes such as the PBS 15 and PBS 80 systems, as well as development of low volume in-situ harvesting methodologies. Other cell lines such as induced pluripotent stem cells (iPSC) and human embryonic stem cells (hES) are also being studied in the Vertical-Wheel™ Bioreactor System.
For this particular application, PBS Biotech generally recommends the MagDrive option of the Vertical-Wheel™ Bioreactor System.
Stem cell therapy research has rapidly grown in interest in recent years, as advanced treatments based on stem cells give the potential to cure a wide variety of serious diseases and infirmities, ranging from inflammation to fixing spinal cord injuries. Numerous clinical products are currently in trial, but the clinical batches to date have mostly been cultured on 2-dimensional systems such as T-flasks, which carry significant economic and regulatory drawbacks for scale up.
One of the scalable approaches for large volume production of stem cells is to grow them on microcarriers (MCs) suspended in a bioreactor as a 3D culture to utilize its entire volume and provide more uniform culturing conditions for the cells to maintain cell homogeneity. The Vertical-Wheel™ Bioreactor System offers an advantage to other bioreactor systems in its ability to keep the MCs in suspension during culture at low agitation rate due to the vertically-oriented wheel, while imparting low shear stress to the cells with gentle mixing owing to the large surface area of the impeller that allows cells to attach quickly and evenly on the MCs.
Bone-Marrow Derived Human Mesenchymal Stem Cells (BM-hMSCs), a commonly-used stem cell type for research and clinical trials, has been successfully cultured in the PBS 3 Vertical-Wheel™ Bioreactor System to very high cell densities:
Scalability of the Vertical-Wheel™ design has also been demonstrated using hMSCs and cell culture medium from Rooster Bio, resulting in consistently high growth rate (1.7-1.8 day doubling time) in PBS 0.5 and PBS 3 systems. The PBS 0.1 and PBS 0.5 MINI Bioreactor systems are ideal for early-stage process development, as they require smaller quantities of expensive reagents to perform screening studies.
PBS Biotech is continually working to generate cutting-edge results in the Vertical-Wheel™ Bioreactor System for Cell Therapy application, including process scale-up to larger working volumes such as the PBS 15 and PBS 80 systems, as well as development of low volume in-situ harvesting methodologies. Other cell lines such as induced pluripotent stem cells (iPSC) and human embryonic stem cells (hES) are also being studied in the Vertical-Wheel™ Bioreactor System.
For this particular application, PBS Biotech generally recommends the MagDrive option of the Vertical-Wheel™ Bioreactor System.